دايافوت_نيكون وتطور التصوير المجهري -٢- مجلة فن التصوير اللبنانية _ ع٣٢

Diafoot_Nikon and the evolution of microscopy_2_

In this second and final episode on microscopy, we learn about an advanced formula for this specialized photographic facility with a diaphragm device from the famous “Nikon” company, which put at the disposal of those interested a device with the utmost perfection and accuracy to raise the level of microscopy to wider areas.

After getting acquainted with microscopic photography as a principle with a regular microscope device, we come to -Diavote -Nikon - the advanced microscopic device to capture the most accurate pictures of the smallest, most moving and active samples.
And we start with a comprehensive and quick look at this device after six and its accessories and features.
⏺ The Diaphot features optical design to make the maximum and most efficient optical path and Nikon human engineering to achieve the highest degree of ease of operation.
⏺ A focusable Bertrand lens was inserted as part of the complex Turret multi-lens imaging tower with the insertion of an optical eyepiece (Veticle) for a photographic mask, and dark slides.
⏺ Accessories include a Cincubator and a Fluorescence System for reflected light. And a device, Numarsky, for different overlapping optical contrast.
⏺Photography, cinematography, and even video viewing are all available in the two implied slots of the camera.
⏺ It can handle different models of holders on a stable platform that stays in place during all focusing work - which makes the diffuser ideal for dealing with microscopic subjects.
⏺ The objective lenses are all new... from the optical contrast stage (4X) to the optical contrast stage (LWD 40X) 20X and 40X with a glass covering zero - 2 mm as a correction ring.

(1) Photography Tower Complex:
The inner part of the telescope body was included with a Bertrand lens for focusing, in order to achieve a quick alignment of the rotating ring, with a drawstring (Phase Annular Ving). The imaging tower was also included in a dark slide location to eliminate stray light coming from the optical system during long-term cinematography, to Low-power focusing magnifying lens side, for ultra-resolution microscopic photography with (2X) and (4X) eyepieces for subjects.

(2) Enlightenment:
Since the Diavote was designed for microscopic photography, it is equipped with a halogen light of 12 volts - 50 watts, which is very effective. For microscopic photography with ordinary daylight films, it is enough to set the voltage for illumination on the “Photo” signal in the voltmeter. For the diaphragm, with the introduction of Nikon color balance screws (10 - NCB).

(3) Endoscope platform and tube:
The body tube of the binoculars has a - Sayed design: Siedentoph adapts quickly to the visual state of the individual viewer without the need to adjust the length of the tube and to provide comfort. This body is inclined by 45 degrees from the horizontal position. As for the peak of visual achievement, it is achieved by removing the additional lens and prismatic lenses that produce unwanted reflections and waste. To the light.
In addition, the platform is adequately located under the head of the endoscope to some extent, in order to ensure easy access and viewing of the sample area.

(4) Fixation and optical path:
Nikon Diavote's engineering focus is on its reliability and functionality. To achieve maximum steadfastness and stability, we find in its molded body a very low center of gravity.
The advanced optical design of the diaphragm “takes advantage of the built-in telescope body and only one reflection surface in the microscope body to increase the optical contrast of the image to the maximum extent, and the conversion of the optical probe from 100% optical to 20% optical - 80% photo is done easily and by one crane, as there is a crane Second, choose the front imaging aperture of a 35 mm camera installed in the interface or the side imaging aperture to make large structures in the field of microscopic photography, microscopic cinematography, and CCTV.

As for the rigid coaxial micro-focusing system, it adjusts the glass slide that holds the subject (the nosepiece) so that the table remains stable, and this is a typical measure for microscopic handling and for greater stability of the sample.

(5) Condenser holder:
The condenser holder can be centered and raised out of the way to accommodate large cylindrical bottles.
It is concentrated by a system of gears and gears (Rack and Pinion) that can withstand heavy work.

(6) The incubator
The Nikon case is an indispensable accessory when it is necessary to adjust the environment. This strong case, made of acrylic plastic, was designed for the divot, in particular, and allows easy access to the sample area, and the temperature control unit uses a hot air circuit to maintain a temperature of 37 degrees Celsius.
There is a separate adjustment knob that allows the temperature to be changed up to 4 degrees Celsius.
A thermometer was also provided with a track for remote temperature detection that could be placed next to the sample.

(7) 35 mm microscopic photography:
The photographic capability is built into the optical system. And to work with the Nikon FE camera, or the “Nikon F3 - the low price,” the diamond can be equipped for microscopic photography, 35 mm automatic exposure.
As for focusing and adjusting the content of microscopic photographs, they are done simply and accurately through the head of the binoculars. There is a grab located directly under the head that brings the eyepiece of the photographic mask to the optical path, and when the image of the sample is brought into precise focus using the eyepiece of the photographic mask, taking the picture becomes possible.

- Accessories
(1) Microscopy film link:
The fully automatic CFMA camera combines a drive motor with automatic exposure adjustment. Moreover, with CFMA, the Bolex camera allows continuous or time-lapse microscopy cinematography along with an automatic exposure implicit readout of the entire image structure.

(2) Large-format microscopic photography: The side opening of the diaphragm accommodates the Nikon (Microflex - FX) series for use with large-format cameras such as 6 x 9 cm, 4 x 5 inches, or Polaroid. No need to re-focus the diaphragm when taking pictures. This is from the Microflex series. The (UFX), (IIFX) and (AFX) models are all adjustable by microaccumulator and feature an automatic exposure system, while the (PFX) is of the manual type.

(3) CCTV link:
Recent improvements in closed-circuit television have increased the popularity of microscopy and the accurate throughput capability of CCTV, ideal for group viewing or video recording, and because of Nikon CF optics, which minimize the axial and lateral chromatic aberrations caused by the lens itself, the image of the subject lens may It is reproduced on the TV screen through the least possible amount of optics: this leads to a sharp, high-contrast image.

(4) Appearance of photocontrast equipment:
The following subject lenses were produced: The unique (CF 4 x DL) graphic low-power achromatic lens.
Achromatic flat objective lens (CF 20 x DL) with correction ring with cover glass 0 - 2 mm : and achromatic flat subject lens for long working distances (CF 40 x LWD) with ring
Correction cover glass zero - 2 mm. These new objective lenses are in addition to what is already included in the Nikon (CF) objective lenses. Achromatic planning and flats.
As for the tower capacitors in these new models, they are designed for the maximum working distance and for ease of operation. The ELWD capacitor has a very long working distance, and it has a working distance of 54 mm and a numerical aperture of 0.32.
As for the LWD capacitor, it has a working distance of 20.5 mm and a numerical aperture of 0.52.

(5) Numarsky DIC:
Nomarsky's interferometric heterochromatic contrast device allows the viewing of non-colored materials or materials
Vivid, by converting the optical path and optical absorption differences of the sample into differences in light contrast or colors, thanks to the advanced design and unique suppression elements in Nikon Numarsky (DIC) that make the device able to provide light contrast and highlight the details in the image. It had never been seen before.
The DIC works with a capacitor (LWD) - numerical aperture 0.52. 20.5 mm working distance - with (10X), (20X) and (40X) locations along with two locations for (10X) and (20X) rings.
In addition to this, there are (4X) and (40X) rings available as accessories.
Finally, we point out that the subject lens (DIC LWD 40X) with a correction package with a cover glass of zero - 2 mm achieves a wonderful achievement with almost any cabin or aquarium.

(6) Radiation of reflected light:
Fluorescence has become a major tool in the laboratory for medical research and analysis. Blending ease of operation and versatility with unimaginably efficient performance, this 100 watt mercury light source delivers the maximum power possible for any radiating application.
As for the units of measurement: (V Modules, BV, and B
And G) which is inserted by sliding and contains spark and obstruction filters and two-color mirrors, which means that the change is easy and fast.
Also, the presence of a zero-density filter allows adjusting the radiation strength to reduce quench while searching for the sample. This is in addition to focusing the lamp in the center quickly and easily by means of a focus target installed on the holder of the glass slide, with the sample to be photographed⏹
Prepared by Dr. Mahmoud Al-Houbi